Analysis of intestinal flora in Mongolian female students under anxiety in a medical university

LI Lian; CHEN Wenting; PANG Hui; BAO Lili; LIU Jia; REN Xiangyu; XU Haixia; BA Teer

doi:10.16835/j.cnki.1000-9817.2022.03.028

Volume 43 Issue 3

Mar. 2022

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LI Lian, CHEN Wenting, PANG Hui, BAO Lili, LIU Jia, REN Xiangyu, XU Haixia, BA Teer. Analysis of intestinal flora in Mongolian female students under anxiety in a medical university[J]. CHINESE JOURNAL OF SCHOOL HEALTH, 2022, 43(3): 433-436. doi: 10.16835/j.cnki.1000-9817.2022.03.028

Citation:

LI Lian, CHEN Wenting, PANG Hui, BAO Lili, LIU Jia, REN Xiangyu, XU Haixia, BA Teer. Analysis of intestinal flora in Mongolian female students under anxiety in a medical university[J]. CHINESE JOURNAL OF SCHOOL HEALTH, 2022, 43(3): 433-436. doi: 10.16835/j.cnki.1000-9817.2022.03.028

Citation:

LI Lian, CHEN Wenting, PANG Hui, BAO Lili, LIU Jia, REN Xiangyu, XU Haixia, BA Teer. Analysis of intestinal flora in Mongolian female students under anxiety in a medical university[J]. CHINESE JOURNAL OF SCHOOL HEALTH, 2022, 43(3): 433-436. doi: 10.16835/j.cnki.1000-9817.2022.03.028

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Analysis of intestinal flora in Mongolian female students under anxiety in a medical university

doi: 10.16835/j.cnki.1000-9817.2022.03.028

LI Lian¹,
CHEN Wenting²,
PANG Hui²,
BAO Lili¹,
LIU Jia³,
REN Xiangyu¹,
XU Haixia^{4
,
,},
BA Teer^{2
,
,}

1.
School of Basic Medicine, Inner Mongolia Medical University, Hohhot(010010), China

Received Date: 2021-09-10
Rev Recd Date: 2021-10-20

Available Online: 2022-03-29

Publish Date: 2022-03-25

Abstract

Abstract

Objective To investigate the change in intestinal flora in Mongolian students with anxiety, so as to provide basis for exploring the relationship between flora and secretion expression in vivo. Methods The Self-rating Anxiety Scale(SAS)was used to assess anxiety in medical college students; then a semi-structured interview was administered. Fecal samples that met the inclusion criteria were collected and divided into anxiety (SAS score≥50) and control groups (no anxiety, SAS score < 50) according to the standard score of SAS. Samples provided by Mongolian female students were selected from each group. The total bacterial DNA was extracted from the fecal samples for PCR amplification and NovaSeq 2x250bp high-throughput sequencing was performed for the V3-V4 region of 16S rDNA gene to obtain the biological information of the intestinal flora. The intergroup OTU, structural diversity, significant difference, and LEfSe analyses were performed with information mining of the literature think tanks. Results Anxiety existed in 23.86% of the Mongolian students, and 16.96% of the Han people. A Chi-square test showed no significant difference in detection of anxiety between Mongolian and Han college students (P=0.07). Analysis of the alpha diversity index showed that the Shannon index, Simpson index, Chao1 index, and Observed species did not differed significantly between the two groups(t=8.0, 9.0, 6.0, 6.5). The difference in abundance of some bacteria was significant at the Class, Order, Family, and Genus levels between the two groups(t=-2.26-2.57, -5.08-3.58, -2.65-2.09, P < 0.05). Conclusion The alpha diversity index showed that there was no significant difference in the abundance and diversity of intestinal flora between the two groups. While there were significant differences at different classification levels, the results suggest that the structure of intestinal flora can change in students with anxiety.
- Anxiety,
- Gastroin testinal tract,
- Bacteria,
- Students,
- Minority groups

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References(17)

References

[1]	CONG X, HENDERSON W A, GRAF J, et al. Early life experience and gut microbiome: the brain-gut-microbiota signaling system[J]. Adv Neonat Care Offic J Nation Assoc Neonat Nurs, 2015, 15(5): 314-323.
[2]	DINAN T G, CRYAN J F. The impact of gut microbiota on brain and behaviour[J]. Curr Opin Clin Nutr Metabol Care, 2015, 18(6): 552-558. doi: 10.1097/MCO.0000000000000221
[3]	ALTHANI A A, MAREI H E, HAMDI W S, et al. Human microbiome and its association with health and diseases[J]. J Cell Physiol, 2016, 231(8): 1688-1694. doi: 10.1002/jcp.25284
[4]	TONG X, XU J, LIAN F, et al. Structural alteration of gut microbiota during the amelioration of human Type 2 diabetes with hyperlipidemia by metformin and a traditional chinese herbal formula: a multicenter, randomized, open label clinical trial[J]. mBio, 2018, 9(3): 1-12.
[5]	NISHINO R, MIKAMI K, TAKAHASHI H, et al. Commensal microbiota modulate murine behaviors in a strictly contamination-free environment confirmed by culture-based methods[J]. Neurogastroenterol Motil, 2013, 25(6): 521-528. doi: 10.1111/nmo.12110
[6]	HOBAN A E, STILLING R M, MOLONEY G M, et al. Microbial regulation of microRNA expression in the amygdala and prefrontal cortex[J]. Microbiome, 2017, 5(1): 102. doi: 10.1186/s40168-017-0321-3
[7]	BUROKAS A, ARBLEYA S, MOLONEY R D, et al. Targeting the microbiota-gut-brain axis: prebiotics have anxiolytic and antidepressant-like effects and reverse the impact of chronic stress in mice[J]. Biol Psychiatry, 2017, 82(7): 472-487. doi: 10.1016/j.biopsych.2016.12.031
[8]	戴晓阳. 常用心理评估量表手册. 北京: 人民军医出版社, 2011: 133-136, 153-155. DAI X Y. Handbook of common psychological assessment scale. Beijing: People's Military Medical Press, 2011: 133-136, 153-155.
[9]	王维. Glo1和CD36介导炎性通路在抑郁症中的作用及机制研究. 重庆: 重庆医科大学, 2020. WANG W. The role and mechanism of Glo1 and CD36 via mediating inflammatory pathway in depression. Chongqing: Chongqing Medical University, 2020.
[10]	ZHANG J C, ZHAO J S, JIN H, et al. Probiotics maintain the intestinal microbiome homeostasis of the sailors during a long sea voyage. Gut Microb, 2020, 11(4): 930-943. doi: 10.1080/19490976.2020.1722054
[11]	WANG K, LIAO M, ZHOU N, et al. Parabacteroides distasonis modulates host metabolism and alleviates obesity and metabolic dysfunctions via production of succinate and secondary bile acids. Cell Rep, 2019, 26(1): 222-235. doi: 10.1016/j.celrep.2018.12.028
[12]	WU F, GUO X, ZHANG J, et al. Phascolarctobacterium faecium abundant colonization in human gastrointestinal tract. Exp Ther Med, 2017, 14(4): 3122-3126. doi: 10.3892/etm.2017.4878
[13]	FIELDING R A, REEVES A R, JASUJA R, et al. Muscle strength is increased in mice that are colonized with microbiota from high-functioning older adults. Exp Gerontol, 2019, 127: 110722. doi: 10.1016/j.exger.2019.110722
[14]	WALKER A, PFITZNER B, HARIR M, et al. Sulfonolipids as novel metabolite markers of Alistipes and Odoribacter affected by high-fat diets. Sci Rep, 2017, 7(1): 11047. doi: 10.1038/s41598-017-10369-z
[15]	张梦洁, 王宁, 袁甜, 等. 蒙古族儿童肠道菌群结构特征分析. 中国学校卫生, 2018, 39(7): 115-118. doi: 10.16835/j.cnki.1000-9817.2018.07.032 ZHANG M J, WANG N, YUAN T, et al. Intestinal flora structure of obese Mongolian children based on DGGE and real-time PCR. Chin J Sch Health, 2018, 39(7): 115-118. doi: 10.16835/j.cnki.1000-9817.2018.07.032
[16]	WU W R, LV L X, SHI D, et al. Protective effect of akkermansia muciniphila against immune-mediated liver injury in a mouse model. Front Microbiol, 2017, 8: 1804. doi: 10.3389/fmicb.2017.01804
[17]	KIM H N, YUN Y J, RYU S, et al. Correlation between gut microbiota and personality in adults: a cross-sectional study. Brain Behav Immun, 2018, 69: 374-385. doi: 10.1016/j.bbi.2017.12.012