Differences in gut microbiota among primary school students with different levels of sugar-sweetened beverage consumption

ZHANG Qin; LIU Xueting; ZHANG Kexin; TIAN Ye; HE Chunlei; WANG Yidi; XU Yujie; SHAN Shufang; WANG Xiaoyu; XIONG Jingyuan; CHENG Guo; HE Fang

doi:10.16835/j.cnki.1000-9817.2024093

Volume 45 Issue 3

Mar. 2024

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Article Navigation > CHINESE JOURNAL OF SCHOOL HEALTH > 2024 > 45(3): 335-340

ZHANG Qin, LIU Xueting, ZHANG Kexin, TIAN Ye, HE Chunlei, WANG Yidi, XU Yujie, SHAN Shufang, WANG Xiaoyu, XIONG Jingyuan, CHENG Guo, HE Fang. Differences in gut microbiota among primary school students with different levels of sugar-sweetened beverage consumption[J]. CHINESE JOURNAL OF SCHOOL HEALTH, 2024, 45(3): 335-340. doi: 10.16835/j.cnki.1000-9817.2024093

Citation:

ZHANG Qin, LIU Xueting, ZHANG Kexin, TIAN Ye, HE Chunlei, WANG Yidi, XU Yujie, SHAN Shufang, WANG Xiaoyu, XIONG Jingyuan, CHENG Guo, HE Fang. Differences in gut microbiota among primary school students with different levels of sugar-sweetened beverage consumption[J]. CHINESE JOURNAL OF SCHOOL HEALTH, 2024, 45(3): 335-340. doi: 10.16835/j.cnki.1000-9817.2024093

Citation:

ZHANG Qin, LIU Xueting, ZHANG Kexin, TIAN Ye, HE Chunlei, WANG Yidi, XU Yujie, SHAN Shufang, WANG Xiaoyu, XIONG Jingyuan, CHENG Guo, HE Fang. Differences in gut microbiota among primary school students with different levels of sugar-sweetened beverage consumption[J]. CHINESE JOURNAL OF SCHOOL HEALTH, 2024, 45(3): 335-340. doi: 10.16835/j.cnki.1000-9817.2024093

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Differences in gut microbiota among primary school students with different levels of sugar-sweetened beverage consumption

doi: 10.16835/j.cnki.1000-9817.2024093

1.
West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu (610041), Sichuan Province, China

Received Date: 2023-11-02
Rev Recd Date: 2024-01-11

Available Online: 2024-03-29

Publish Date: 2024-03-25

Abstract

Abstract

Objective To explore the differences in the gut microbiota of primary school students with different levels of sugar-sweetened beverage intake, so as to provide scientific evidence for better identification of health risks in children and the development of targeted health policies. Methods In June 2022, a total of 192 healthy primary school students from Chengdu were selected using a stratified cluster random sampling method. The sugar-sweetened beverage intake was assessed through a dietary frequency questionnaire. Based on the median daily sugar-sweetened beverage intake, primary school students were categorized into a low-intake group (n=96) and a high-intake group (n=96). The gut microbiota in fresh fecal samples from the two groups of primary school students was analyzed using 16S rRNA high-throughput sequencing, and the diversity and community structure differences in the gut microbiota were compared. Results Children in the low-intake group had a sugar-sweetened beverage intake of (21.3±1.6) mL/d, while the high-intake group had an intake of (269.6±37.3) mL/d. Diversity analysis results showed that there were no statistically significant differences between the low-intake and the high-intake group in terms of α diversity metrics: Observed_otus index [298.50 (259.75, 342.25), 305.50 (244.25, 367.75)], Goods_coverage index [1.00 (1.00, 1.00), 1.00 (1.00, 1.00)], Chao index [304.18 (260.75, 348.78), 305.88 (245.68, 370.88)], Shannon index [5.88 (5.29, 6.45), 5.71 (4.89, 6.28)] and Simpson index [0.95 (0.91, 0.97), 0.94 (0.88, 0.97)] (Z=-0.64, -0.76, -0.54, -1.76, -1.67, P>0.05). Furthermore, no statistically significant difference was observed in β diversity between the two groups (R²=0.006, P>0.05). At the genus level, the abundance of Blautia [0.033 (0.018, 0.055)] and Fusicatenibacter [0.009 (0.005, 0.015)] were higher in the low-intake group compared to the high-intake group [0.024 (0.013, 0.041), 0.006 (0.003, 0.011)]and differences were statistically significant (Z=-2.52, -2.81, P < 0.05). LEfSe analysis highlighted intergroup differences primarily in Blautia, Fusicatenibacter and Sarcina(LDA=3.56, 3.12, 3.53, P < 0.05). Conclusions There is no significant difference in the diversity and overall structure of the gut microbiota in primary school students with different levels of sugar-sweetened beverage intake. However, there are species variations at the genus level. The information can serve as a scientific basis for identifying health risks in primary school students and formulating targeted health strategies.
- Carbohydrates,
- Beverages,
- Feeding behavior,
- Gastrointestinal tract,
- Bacteria,
- Child

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References(40)

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