The prevalence of carrier status of pathogens of Hand Foot and Mouth disease among healthy children in Chengdu kindergartens in different periods

Objective This study aimed to analyze the prevalence of carrier status of pathogens of Hand Foot and Mouth disease among healthy children in Chengdu kindergartens in different periods and to provide a scientific reference for developing strategies for the prevention and control of HFMD.Methods Random sampling method was used to select 758 healthy children from 120 kindergartens in Chengdu,and they were tested with real-time PCR to get the information on enteroviruses during the period of highs and lows.Results The positive rate of enterovirus in healthy children was 8.93% in epidemic period,and the positive rate was 2.13% in non-epidemic period.The positive rate in epidemic period was higher than that in non-epidemic period(x2 =17.70,P<0.01).The positive rate of other enteroviruses in epidemic period was 7.44% which was higher than that in non-epidemic period(x2 =12.30,P<0.01).The positive rate of enterovirus among healthy children in urban was higher than that in suburbs in epidemic period(x2 =10.47,P=0.001).The incidence of other enteroviruses in healthy children was higher than that in the confirmed cases (x2=28.26,P<0.01).The rates of CA16 and EV71 were lower than those in the confirmed cases(P<0.01).Conclusion In epidemic periods we should strengthen the prevention and control work of HFMD in urban,carry out pathogen surveillance on other enteroviruses,and focus on the prevalence of EV71 carrying among healthy children in Chengdu.