学校食堂餐饮食品加工过程致病性微生物监测结果

牛会敏; 焦强; 李若菡; 刘智勇

doi:10.16835/j.cnki.1000-9817.2024194

学校食堂餐饮食品加工过程致病性微生物监测结果

doi: 10.16835/j.cnki.1000-9817.2024194

河南省食品和盐业检验技术研究院/国家市场监管重点实验室(食品安全快速检测与智慧监管技术)，郑州 450003

基金项目:

2021年度国家市场监督管理总局科技计划项目 2021MK065

详细信息

作者简介:
牛会敏(1985-)，女，河南省人，硕士，工程师，主要研究方向为食品微生物学及分子生物学检验

通讯作者:
刘智勇，E-mail：liuzhiyong9@126.com

利益冲突声明 所有作者声明无利益冲突。
中图分类号: R627.8 R155 Q93-331
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- 被引次数: 0
出版历程
- 收稿日期: 2024-03-13
- 修回日期: 2024-04-15
- 网络出版日期: 2024-06-27
- 刊出日期: 2024-06-25

Pathogenic microorganism monitoring in the food processing of school canteens

Henan Institute of Food and Salt Industry Inspection Technology/Key Laboratory of Food Safety Quick Testing and Smart Supervision Technology for State Market Regulation, Zhengzhou (450003), Henan Province, China

摘要

摘要: 目的了解学校食堂餐饮食品加工过程中致病性微生物污染的风险点，为预防校园食品安全事件的发生提供参考。方法于2023年3月，采用方便抽样方法抽取河南省郑州市10所学校食堂为研究对象，采集学校食堂在餐饮食品加工过程中原料贮存设备、制作工具、工作人员手部、基础设施、用餐器具等共300份样品，利用基于等温多自配引发扩增技术(IMSA)建立的食源性致病菌快速检测方法分析可能存在的致病性微生物污染的风险。采用Fisher确切概率法进行统计学分析。结果 300份样品中, 共有5份样品检出了包括金黄色葡萄球菌、沙门菌、单核细胞增生李斯特菌在内的3种致病性微生物，主要分布在食品制作过程的工具如砧板、操作台、刀具以及工作人员的手部，而用餐器具、原料贮存设备及食堂基础设施均未检出致病性微生物。IMSA快速检测方法和国标方法对金黄色葡萄球菌、沙门菌、单核细胞增生李斯特菌的检验结果差异无统计学意义(P>0.05)。与国标方法相比，IMSA快检系统检测致病性微生物的阳性符合率为83.3%(5/6)，阴性符合率为100%(294份)，整体一致性为99.7%。结论利用IMSA技术建立的致病性微生物快速检测方法，可精准识别出学校食堂餐饮食品加工过程中致病性微生物污染的风险点主要为食品制作过程的工具如砧板、操作台、刀具以及工作人员的手部。应提高校园食品安全监管的质量和效率，预防校园食品安全事件的发生。
- 食品处理和加工 /
- 食品微生物学 /
- 食品污染 /
- 监测 /
- 学生保健服务
Abstract: Objective To analyze the key control points of pathogenic microbial contamination during the food processing of school canteens, so as to provide a basis for effectively preventing the occurrence of campus food safety incidents. Methods In March 2023, 10 school canteens from Zhengzhou City, Henan Province were selected by convenient sampling method. A total of 300 samples were collected from the storage equipment of raw materials, production tools, staff hands, infrastructure, dining utensils and other facilities in the school canteens during the food processing process.The rapid detection method of foodborne pathogens based on isothermal multiple self-matching-initiated amplification (IMSA) technology was used to analyze the possible risk of pathogenic microorganism contamination. Fisher exact probability method was applied for the statistical analysis. Results Among the 300 samples collected, pathogenic microorganisms including Staphylococcus aureus, Salmonella and Listeria monocytogenes were detected in 5 samples, distributed in the food preparation utensils such as cutting boards, operating tables, knives and staff hands, while no pathogenic microorganisms were detected in dining utensils, raw material storage equipment and canteen infrastructure.There was no statistically significant difference between the IMSA rapid detection method and the national standard method for testing Staphylococcus aureus, Salmonella and Listeria monocytogenes(P>0.05). Compared to international method, the positive coincidence of pathogenic microorganism detected by IMSA rapid inspection system was 83.3%(5/6), the negative coincidence was 100%(n=294), and the overall consistency rate was 99.7%. Conclusions The key control points of pathogenic microbial contamination in the food processing process of school canteens mainly are the utensils in the food production process, such as cutting boards, operating tables, knives and staff hands in the food processing process of the school canteen, which could be accurately identified by the rapid detection method of pathogenic microorganism established based on IMSA technology. The quality and efficiency campus food safety supervision should be promoted to prevent the food safety events in school canteen.
- Food handling /
- Food microbiology /
- Food contamination /
- Monitoring /
- Student health services
注释:

1) 利益冲突声明 所有作者声明无利益冲突。

HTML全文

表 1 不同检测项目两种方法致病性微生物检测结果

Table 1. Test results of pathogenic microorganisms fordifferent testing items by two methods

检测项目	两方法结果均为阴性	两方法结果均为阳性	国标结果阳性，快检结果阴性	国标结果阴性，快检结果阳性
金黄色葡萄球菌	99	1	0	0
沙门菌	97	3	0	0
单增李斯特菌	98	1	0	1
总计	294	5	0	1
注：IMSA快速检测简称“快检”。

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表 2 不同学校检出阳性采样点及致病性微生物分布

Table 2. Distribution of detected positive sampling points and pathogenic microorganism among different schools

学校编号	采样点	检测项目	快检结果	国标结果
1	砧板	单增李斯特菌	+	+
1	操作台	金黄色葡萄球菌	+	+
8	工作人员的手部	沙门菌	+	+
9	刀	沙门菌	+	+
9	操作台	沙门菌	+	+

下载: 导出CSV

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