最大脂肪氧化强度运动和饮食限制对肥胖非酒精性脂肪肝病大学生血脂及铁死亡的影响

刘炎冰; 李巧娥; 门杰; 施文海

doi:10.16835/j.cnki.1000-9817.2024182

最大脂肪氧化强度运动和饮食限制对肥胖非酒精性脂肪肝病大学生血脂及铁死亡的影响

doi: 10.16835/j.cnki.1000-9817.2024182

1.
山西医科大学汾阳学院体育教研室，汾阳 032200
2.
汾阳市人民医院消化内科
3.
太原师范学院体育学院

基金项目:

2022年山西省教育科学“十四五”规划课题 GH-220343

山西医科大学汾阳学院科研基金项目 2017A01

详细信息

作者简介:
刘炎冰(1981-)，男，山西汾阳人，硕士，讲师，主要研究方向为体育教育与运动康复

通讯作者:
施文海，E-mail: tysyswh@163.com

利益冲突声明 所有作者声明无利益冲突。
中图分类号: R179 G806 R723.14 R589.2
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- 被引次数: 0
出版历程
- 收稿日期: 2024-02-20
- 修回日期: 2024-03-29
- 网络出版日期: 2024-06-27
- 刊出日期: 2024-06-25

Effects of maximum fat oxidation intensity exercise combined with diet restriction on lipid and ferroptosis in obese college students with non-alcoholic fatty liver disease

1.
Department of Sports, Fenyang College of Shanxi Medical University, Fenyang(032200), Shanxi Province, China

摘要

摘要: 目的比较12周最大脂肪氧化(FATmax)强度运动结合饮食限制及单独FATmax强度运动对肥胖非酒精性脂肪性肝病(NAFLD)大学生身体成分、脂代谢及铁死亡的影响，为肥胖NAFLD大学生的康复治疗提供运动处方参考。方法于2023年8月，通过网上发布通知和山西医科大学汾阳学院健康体检中心招募在校本科生，从中选取45名肥胖NAFLD大学生，用随机数字表法分为FATmax强度运动结合饮食限制组(FATmax+DR，22名)和FATmax强度运动组(FATmax组，23名)。其中FATmax组进行12周、每周3次的FATmax强度运动(FATmax: 0.51 g/min，运动强度: 50.14% VO₂max，对应的心率: 136.78次/min)；FATmax+DR组进行12周FATmax强度运动结合饮食限制(每日饮食摄入能量依据静息能耗确定食谱)。干预前后分别对两组受试者身体成分、血脂4项及氧化应激、铁死亡相关指标进行测试。采用独立样本t检验及配对样本t检验分析各组指标差异。结果干预前，FATmax+DR组和FATmax组身体成分、血脂4项及氧化应激、铁死亡相关指标差异均无统计学意义(P值均>0.05)。干预12周后，FATmax+DR组体重、体质量指数(BMI)、体脂肪量、躯干脂肪量、内脏脂肪面积及血清低密度脂蛋白(LDL)、丙二醛(MDA)和铁蛋白水平均低于FATmax组，血清高密度脂蛋白(HDL)及谷胱甘肽(GSH)水平均高于FATmax组(t值分别为-2.30，-3.23，-3.97，-5.85，-3.44，-3.06，-2.03，-2.09；2.73，2.42，P值均 < 0.05)；两组间血清总胆固醇(TC)、甘油三酯(TG)、超氧化物歧化酶(SOD)及谷胱甘肽过氧化物酶4(GPX4)水平差异均无统计学意义(t值分别为1.49，1.00，-0.01，0.59，P值均>0.05)。结论 12周FATmax强度运动结合饮食限制在改善肥胖NAFLD大学生身体成分及血脂紊乱、抑制铁死亡发生及肝脏氧化应激损伤方面的效果更加显著。
- 运动活动 /
- 膳食 /
- 脂肪肝 /
- 脂类代谢 /
- 干预性研究 /
- 学生
Abstract: Objective To compare the effects of 12-week maximum fat oxidation (FATmax) intensity exercise combined with dietary restriction and FATmax intensity exercise alone on body composition, blood lipid metabolism and ferroptosis in obese college students with non-alcoholic fatty liver disease(NAFLD), so as to provide a exercise prescription reference for rehabilitation treatment of obese NAFLD college students. Methods In August 2023, a total of 45 obese NAFLD college students were divided into maximum fat oxidation intensity exercise combined with dietary restriction group (FATmax+DR, n=22) and FATmax group (n=23) by random number table method, who were recruited through online notifications and physical examinations at the Physical Examination Center of Fenyang College of Shanxi Medical University. FATmax group received FATmax intensity exercise with 3 times a week for 12 weeks (FATmax: 0.51 g/min, exercise intensity: 50.14% VO₂max and the heart rate: 136.78 times/min). FATmax+DR group underwent 12 weeks of FATmax intensity exercise combined with dietary restriction, and daily dietary energy intake was determined according to resting energy consumption. Body composition, four items of blood lipids and the indicators of oxidative stress and ferroptosis were measured before and after the intervention.The differences of indicators among each group were analyzed by using independent sample t test and paired sample t test. Results Before the intervention, there were no significant differences in body composition, four items of blood lipid, oxidative stress and iron death between FATmax+DR group and FATmax group (P>0.05). After 12 weeks of intervention, body weight, body mass index(BMI), body fat mass, trunk fat mass, visceral fat area and serum low density lipoprotein(LDL) and malondialdehyde(MDA) levels in FATmax+DR group were significantly lower than those in FATmax group, while serum high density lipoprotein(HDL) and glutathione(GSH) levels were higher than those in FATmax group (t=-2.30, -3.23, -3.97, -5.85, -3.44, -3.06, -2.03, -2.09; 2.73, 2.42, P < 0.05). There were no significant differences in serum levels of total cholesterol(TC), triglyceride(TG), superoxide dismutase(SOD) and glutathion peroxidase 4(GPX4) between the two groups (t=1.49, 1.00, -0.01, 0.59, P>0.05). Conclusion Twelve-week FATmax intensity exercise combined with dietary restriction could significantly improve body composition and dyslipidemia, prevent the occurrence of ferroptosis and liver oxidative damage in obese NAFLD college students, which could serve as a timely promotion and application of intervention for obese college students with NAFLD.
- Motor activity /
- Diet /
- Fatty liver /
- Lipid metabolism /
- Intervention studies /
- Students
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表 1 不同组别干预前后肥胖NAFLD大学生身体成分比较(x±s)

Table 1. Comprison of body composition of obese NAFLD college students in different groups before and after intervention(x±s)

组别	干预前后	人数	统计值	体重/kg	BMI/(kg·m^-2)	体脂肪量/kg	躯干脂肪量/kg	内脏脂肪面积/cm²
FATmax组	干预前	23		81.74±5.41	29.84±1.41	29.20±2.13	17.15±1.23	115.30±4.40
	干预后	23		79.50±5.68	28.65±1.27	27.45±1.89	16.99±1.31	112.61±4.54
			t值	5.46	5.36	8.56	1.87	8.19
			P值	< 0.01	< 0.01	< 0.01	0.08	< 0.01
FATmax+DR组	干预前	22		80.42±5.09	29.47±1.39	28.82±1.58	16.59±1.35	114.91±4.26
	干预后	22		75.78±5.16	27.54±1.02	25.29±1.75	14.52±1.52	107.86±4.70
			t值	15.48	14.63	13.11	9.55	9.45
			P值	< 0.01	< 0.01	< 0.01	< 0.01	< 0.01

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表 2 不同组别干预前后肥胖NAFLD大学生血脂水平比较(x±s，mmol/L)

Table 2. Comparison of blood lipid levels of obese NAFLD college students in different groups before and after intervention(x±s, mmol/L)

组别	干预前后	人数	统计值	TC	TG	HDL	LDL
FATmax组	干预前	23		4.92±0.93	2.13±0.51	1.18±0.31	3.59±0.54
	干预后	23		4.80±0.90	2.03±0.47	1.24±0.20	3.50±0.46
			t值	1.88	2.61	-2.24	1.74
			P值	0.07	0.02	0.04	0.10
FATmax+DR组	干预前	22		4.75±0.96	2.04±0.63	1.16±0.18	3.44±0.37
	干预后	22		4.44±0.73	1.89±0.52	1.45±0.20	3.09±0.33
			t值	1.74	2.21	-11.26	6.67
			P值	0.10	0.04	< 0.01	< 0.01

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表 3 不同组别干预前后肥胖NAFLD大学生铁死亡及氧化应激指标比较(x±s)

Table 3. Comparison of ferroptosis and oxidative stress indicators of obese NAFLD college students in different groups before and after intervention(x±s)

组别	干预前后	人数	统计值	SOD/(U·mL^-1)	MDA/(nmol·mL^-1)	GSH/(μmol·L^-1)	GPX 4/(ng·mL^-1)	血清铁蛋白/(μg·L^-1)
FATmax组	干预前	23		70.81±12.84	6.57±1.47	114.31±14.58	64.06±10.18	121.79±12.68
	干预后	23		73.48±11.43	5.88±1.13	120.87±15.55	65.03±0.98	117.04±11.65
			t值	-5.28	8.09	-6.15	-2.04	6.01
			P值	< 0.01	< 0.01	< 0.01	0.05	< 0.01
FATmax+DR组	干预前	22		68.21±9.53	6.31±1.19	119.61±10.93	62.39±8.95	116.17±12.74
	干预后	22		73.51±7.36	5.23±1.01	130.11±9.40	63.43±8.04	110.12±10.46
			t值	-8.70	12.29	-10.79	-2.97	6.67
			P值	< 0.01	< 0.01	< 0.01	< 0.01	< 0.01

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