健康大学生多环芳烃内暴露与肺功能变化的关系

陈俊杰; 刘思巧; 李淼; 郝珂璐; 张艳丽; 李卫红; 徐厚君; 郝玉兰; 王曼曼; 王学生; 王茜

doi:10.16835/j.cnki.1000-9817.2023.09.029

健康大学生多环芳烃内暴露与肺功能变化的关系

doi: 10.16835/j.cnki.1000-9817.2023.09.029

1.
华北理工大学公共卫生学院，河北唐山 063000
2.
华北理工大学心理与精神卫生学院
3.
华北理工大学实验动物中心

基金项目:

河北省自然科学基金面上项目 H2021209054

河北省自然科学基金面上项目 H2022209025

唐山市科技计划项目 19130209g

详细信息

作者简介:
陈俊杰(1998-), 男, 广东汕尾人, 在读硕士, 主要研究方向为环境有害因素与健康

通讯作者:
王茜, E-mail: wwqq517@aliyun.com

利益冲突声明 所有作者声明无利益冲突。
中图分类号: R816.41 R179 R193
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- 被引次数: 0
出版历程
- 收稿日期: 2023-06-14
- 修回日期: 2023-06-27
- 网络出版日期: 2023-09-28
- 刊出日期: 2023-09-25

Relationship between polycyclic aromatic hydrocarbons internal exposure and lung function change among healthy college students

1.
School of Public Health, North China University of Science and Technology, Tangshan (063000), Hebei Province, China

摘要

摘要: 目的研究尿中单羟基多环芳烃代谢物(OH-PAHs)与肺功能之间的关系以及氧化应激在其中的中介作用，为空气污染治理与政策制定提供科学依据。方法招募华北理工大学45名健康大学生为研究对象，分别于2017年11月至2018年10月进行4次随访调查和健康体检，测量肺功能参数[用力肺活量(FVC)、1 s用力呼气量(FEV1)、呼气峰值流速(PEF)、FEV1/FVC和用力呼气流量(FEF_25%~75%)]和尿液中7种暴露标志物OH-PAHs(∑₇OH-PAHs)、氧化应激标志物8-羟基-2'-脱氧鸟苷(8-OHdG)、8-异前列腺素F_2α(8-iso-PGF_2α)。利用线性混合效应模型分析尿多环芳烃代谢物与肺功能之间的关系，并用中介模型评估氧化应激在OH-PAHs与肺功能之间关系中的中介效应。结果研究对象FVC、FEV1、FEV1/FVC、PEF和FEF_25%~75%的中位数分别为4.37 L，3.58 L，83.00%，4.38 L/s和3.32 L/s。2-羟基芴(2-OHFlu)的对数转换值每增加1个(ln)单位，FVC下降5.05%(β%=-5.05%，95%CI=-8.85%~-1.09%)，FEV1下降4.15%(β%=-4.15%，95%CI=-7.94%~-0.22%)，FEF_25%~75%下降5.87%(β%=-5.87%，95%CI=-11.35%~-0.05%)。2-OHFlu和9-羟基菲(9-OHPhe)的对数转换值每增加1个(ln)单位，PEF分别降低7.03%(β%=-7.03%，95%CI=-12.60%~-1.11%)和7.08%(β%=-7.08%，95%CI=-13.50%~-0.17%)。此外，尿∑₇OH-PAHs与尿8-OHdG和8-iso-PGF_2α水平呈正相关(r值分别为0.64，0.69，P值均＜0.01)。同时，8-OHdG介导2-OHFlu与FVC和FEV1的相关性分别为17.06%和15.71%。结论健康大学生尿中OH-PAHs与肺功能呈负相关，8-OHdG在2-OHFlu与FVC和FEV1之间的关系中起介导作用。应积极制定相关政策治理空气污染，维护青年人的健康生活状态。
- 多环芳烃 /
- 肺 /
- 氧化性应激 /
- 学生 /
- 线性模型
Abstract: Objective To investigate the relationship between urinary monohydroxylated metabolites of hydroxyl polycyclic aromatic hydrocarbons (OH-PAHs) and lung function, as well as the role of oxidative stress in these associations, so as to provide a scientific basis for air pollution control and policy formulation. Methods A panel study was carried out among 45 young healthy adults. Four follow-up surveys and health examinations were conducted from November 2017 to October 2018 to measure lung function parameters [forced vital capacity (FVC), second forced expiratory volume in one second (FEV1), peak expiratory flow (PEF), FEV1/FVC, and forced expiratory flow between 25% and 75% vital capacity (FEF_25%~75%)], markers of exposure to 7OH-PAHs [∑₇OH-PAHs], and markers of oxidative stress[8-hydroxy-2'-deoxyguanosine (8-OHdG) and 8-isoprostaglandin-F_2α (8-iso-PGF_2α)]. The relationship between urinary PAH metabolites and lung function was quantified by linear mixed effects models. Mediation analysis was performed to assess the role of oxidative stress in the relationship between OH-PAHs and lung function. Results The median values of FVC, FEV1, FEVI/FVC, PEF, and FEF_25%-75% were 4.37 L, 3.58 L, 83.00%, 4.38 L/s, and 3.32 L/s, respectively. The results showed that each 1-unit increase in log-transformed value of 2-Hydroxyfluorene (2-OHFlu) was associated with a 5.05% decrease (β%=-5.05%, 95%CI=-8.85%--1.09%) in FVC, 4.15% decrease (β%=-4.15%, 95%CI=-7.94%--0.22%) in FEV1 and 5.87% decrease (β%=-5.87%, 95%CI=-11.35%--0.05%) in FEF_25%-75%, respectively. Each 1-unit increase in log-transformed values of 2-OHFlu and 9-Phenanthrol (9-OHPhe) was associated with a 7.03% decrease (β%=-7.03%, 95%CI=-12.60%--1.11%) and a 7.08% decrease (β%=-7.08%, 95%CI=-13.50%--0.17%) in PEF, respectively. Additionally, urinary ∑₇OH-PAHs had a positive correlation with the levels of urinary 8-OHdG and 8-iso-PGF_2α (r=0.64, 0.69, P < 0.01). Meanwhile, the levels of 8-OHdG mediated 17.06% and 15.71% of the association between 2-OHFlu with FVC and FEV1. Conclusion The finding reveales a negative relationship between urinary OH-PAHs and lung function among young healthy adults. The 8-OHdG plays a mediated role in the correlation of 2-OHFlu with FVC and FEV1. Active relevant policies are needed to control air pollution and maintain the healthy living conditions of young people.
- Polycyclic aromatic hydrocarbons /
- Lung /
- Oxidative stress /
- Students /
- Linear models
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表 1 不同季节肺功能参数、尿中OH-PAHs及氧化应激标志物水平比较[M(IQR)，n=45]

Table 1. Comparison of levels of lung function parameters, OH-PAHs and oxidative stress markers in urine in different seasons[M(IQR), n=45]

季节	FVC/L	FEV1/L	FEV1/FVC/(%)		PEF/(L·s^-1)	FEF_25%~75%/(L·s^-1)	2-OHNap^a	1-OHNap^a	2-OHFlu^a
冬	4.15(2.01)	1.04(0.67)	88.00(6.00)		1.18(0.79)	0.91(0.76)	0.94(1.25)	2.41(2.70)	0.44(0.68)
春	5.00(1.43)	3.74(1.06)	80.00(9.00)		5.16(1.67)	3.79(1.24)	0.45(0.97)	2.36(2.46)	0.35(0.65)
夏	3.14(1.33)	3.52(1.20)	86.00(9.00)		2.97(1.47)	2.42(1.29)	0.32(0.94)	2.36(2.33)	0.53(0.61)
秋	4.79(0.83)	3.64(0.89)	80.00(7.00)	4.38(1.62)		3.34(1.08)	0.35(0.66)	2.16(2.42)	0.41(0.58)
H值	61.72	45.30	25.47		47.61	35.15	19.35	3.58	4.70
P值	＜0.01	＜0.01	＜0.01		＜0.01	＜0.01	＜0.01	0.31	0.20

季节	3-OHPhe^a	9-OHPhe^a		1-OHPhe^a		1-OHPyr^a	∑₇OH-PAHs^a	8-OHdG^a	8-iso-PGF_2α^a
冬	0.11(0.12)	0.47(0.56)		0.34(0.43)		4.70(4.55)	11.04(10.80)	20.27(22.02)	0.18(0.13)
春	0.08(0.11)	0.29(0.45)		0.20(0.25)		4.88(4.62)	8.60(10.66)	20.29(23.36)	0.16(0.26)
夏	0.09(0.09)	0.51(0.57)		0.27(0.42)		5.71(4.48)	10.61(10.09)	15.18(22.84)	0.08(0.11)
秋	0.08(0.13)	0.41(0.58)		0.22(0.46)		5.21(8.64)	10.61(13.17)	11.08(15.03)	0.06(0.07)
H值	2.67	6.72		7.75		2.49	1.15	12.63	50.70
P值	0.45	0.08		0.05		0.48	0.77	0.01	＜0.01
注：a单位为μg/g Cr。

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表 2 氧化应激在尿多环芳烃代谢物与肺功能关系中的介导作用

Table 2. Mediating effect of oxidative stress(8-OHd Gand 8-iso-PGF_2α) on the associations between urinary OH-PAHs and lung function parameters

OH-PAHs	中介变量	因变量	总效应(95%CI)	介导效应(95%CI)
2-OHFlu	8-OHdG	FVC	-0.05(-0.09~-0.01)^*	-0.01(-0.02~0.00)
	8-OHdG	FEV1	-0.04(-0.08~0.00)^*	-0.01(-0.03~0.00)
	8-OHdG	PEF	-0.07(-0.12~-0.02)^*	-0.00(-0.02~0.01)
	8-OHdG	FEF_25%~75%	-0.05(-0.10~0.00)^*	-0.01(-0.02~0.01)
	8-iso-PGF_2α	FVC	-0.08(-0.12~-0.03)^*	0.00(-0.01~0.01)
	8-iso-PGF_2α	FEV1	-0.07(-0.12~-0.02)^*	0.00(-0.01~0.01)
	8-iso-PGF_2α	PEF	-0.09(-0.14~-0.04)^*	0.00(-0.01~0.01)
	8-iso-PGF_2α	FEF_25%~75%	-0.07(-0.15~0.00)^*	0.00(-0.01~0.01)
9-OHPhe	8-OHdG	FVC	-0.03(-0.07~0.01)	-0.01(-0.03~0.00)
	8-OHdG	FEV1	-0.02(-0.06~0.03)	-0.01(-0.03~0.00)
	8-OHdG	PEF	-0.06(-0.11~0.01)	-0.01(-0.03~0.02)
	8-OHdG	FEF_25%~75%	-0.02(-0.08~0.04)	-0.01(-0.03~0.01)
	8-iso-PGF_2α	FVC	-0.03(-0.08~0.02)	0.00(-0.02~0.01)
	8-iso-PGF_2α	FEV1	-0.02(-0.07~0.03)	0.00(-0.01~0.01)
	PEF	-0.03(-0.11~0.04)	-0.01(-0.03~0.01)
	8-iso-PGF_2α	FEF_25%~75%	0.00(-0.07~0.07)	-0.01(-0.03~0.00)
注：*P＜0.05。

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