Abstract: Objective To evaluate the effects of hand hygiene interventions in kindergartens in Luohu area,and to provide a reference for the supervising of having a good hand hygiene habit among children.Methods A total of 3 886 children from 119 classes from 10 kindergartens in Luohu area in Shenzhen were randomly selected,and all the children (3 886) in the selected kindergartens received integrated interventions.HH compliance of children were separately observed before and after the implementation of interventions,and the cases of hand-foot-and-mouth disease of children in all kindergartens in Luohu district were collected and compared for the effect evaluation.Results After the implementation of interventions,the HH compliance of children in selected kindergartens was significantly improved (RR =2.135,95% CI =1.885-2.417),and the most significant improvement was found in the senior class(RR=2.559,95%CI=2.134-3.068) and before preparing food(RR=2.877,95%CI=1.700-4.870).The results of multivariable Logistic regression analysis indicated that reminding teachers of washing hands (OR =14.765,95% CI =12.244-17.805) was the most important determinant of HH compliance.However,the incidence of hand-foot-and-mouth disease was not significantly decreased in selected kindergartens(RR =0.866,95%CI=0.622-1.207).Conclusion The implementation of interventions can effectively improve HH compliance of children in kindergartens.