Abstract: Objective To explore the relationship between physical fitness test and muscle parameter among male college students in Guangxi.Methods A total of 607 male college students were randomly selected,whose muscle nass were measured by professional Body Composition Monitors (Tanita MC-180).According to the national standard for physical health test,vital capacity,50 meters dash,1 000-meters run,sit and reach test,standing long jump and pull-ups.Data were analyzed by SPSS 20.0 software.Results Body mass index(BMI),vital capacity,lung capacity showed significant differences in students with different muscle volume(F=228.415,10.794,40.245,P<0.01).Average BMI and vital capacity for male students was highest among high muscle group,followed by moderate and low group.Mean value of lung capacity relative body mass index was highest among low muscle group,followed by moderate and high group.Significant differences in standing long jump,sit and reach test and pull up were found in male college students with different muscle mass(F=4.036,4.027,52.250,P<0.05),and average values of standing long jump and sit and reach test were highest in moderate muscle mass group,followed by low and high group.Average values of pull up was higher in low and high muscle group than medium muscle group(P<0.05).The 50 m dash and 1 000 m run scores showed no significant differences among male students with different muscle mass.Conclusion The amount of muscle mass correlate with physical fitness among male college students.Maintaining suitable muscle mass and enhancing muscular strength would help to improve physical fitness among college students.