Abstract: Objective To investigate knowledge and attitude on Dengue among primary and secondary school students of Guangzhou in 2015,aiming to provide evidence for future prevention.Methods By using multi-stage cluster sampling method,elementary and secondary school students of grade 3,5,8,11 in Guangzhou were investigated by self-administered questionnaires and the results were analyzed with descriptive statistics.Results The top three awareness on Dengue were:"emptying ponding is a way to prevent mosquitoes from growth"(91.8%),"fever is one of the common early symptoms of Dengue fever"(91.4%),and "mosquitoes spreading dengue fever are black with white stripes"(89.5%).The lowest items of awareness on Dengue were:"filling containers such as hollow bamboo joints is a way to prevent mosquitoes from growth(35.4%),mosquitoes eggs can grow into mosquitoes larvae after staying in the water for 3 days"(29.5%),"when hydrophyte is kept,breeding fish in the bottle can prevent mosquitoes larvae from growth"(23.6%).The median self-rating on possibility of suffering dengue fever was 2 (range:1-3) (up to 5),and the median self-rating on health risk after suffering dengue fever was 4(range:3-5),the median self-rating on cooperation with family removing mosquito breeding sites was 3(range:2-4).All the three self-rating score significantly differed in grade and regions.Conclusion After Dengue outbreak in Guangzhou,the awareness of Dengue among primary and secondary school students still needs to be substantially improved.Self-perceived attitude or believes might be helpful for targeted health education,especially among students in different grade and regions.