摘要:
了解小学生携带甲氧西林耐药金葡菌(MRSA)情况及菌株中毒素基因,为靶向性药物研发等医学实践提供遗传标志物资料.方法 方便整群抽取广州市l所小学在校健康小学生1 012名,进行鼻拭子采样,采用PCR实验检测菌株中毒素基因.结果 小学生中金葡菌携带率为40.1%,MRSA携带率为1.2%.金葡菌中hlb,sak,scn,chp,sep基因的检出率较高(81.7%,97.8%,82.4%,77.0%),其次是seA,seB,seC基因(33.4%,15.1%,7.2%).MRSA菌株中的chp,seB,seC,pvl基因的检出率均明显高于甲氧西林敏感金葡菌(MSSA)菌株(P值均<0.05);MRSA菌株中同时检出2种或3种毒素基因的检出率均明显高于MSSA菌株(P值均<0.05).结论 健康小学生金葡菌及其毒素基因携带率较高,MRSA菌株的潜在致病能力不容忽视.
Abstract:
Objective To determine the molecular epidemiology and presence of virulence genes in Staphylococcus aureus (S.aureus) isolates from primary school students in Guangzhou.Methods Nasal swab samples were collected from healthy primary school students in Guangzhou.The polymerase chain reaction (PCR) technique was applied to test the virulence genes from S.aureus strains.Results Overall,40.1% and 1.2% of the isolates were classified as S.aureus and methicillin-resistant S.aureus (MRSA) among 1 012 primary school students,respectively.The leading toxin genes were hlb,sak,scn,chp and sep genes in S.aureus,followed by seA,seB and seC genes.The frequency of chp,seB,seC and pvl genes was higher in MRSA than in methicillin-susceptible S.aureus (MSSA) (P<0.05).The coexistence of two or three virulence genes was higher in MRSA than in MSSA(P <0.05).Conclusion The relative high frequency of S.aureus and its virulence genes among healthy primary school students in Guangzhou warrants further attention considering the potential pathogenic ability of MRSA.
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