Abstract: Objective To understand the current situation of tuberculosis prevention and control in middle school and college,and to provide evidence for further improvement of the strategy of school tuberculosis prevention and control.Methods Selfdesigned questionnaires were used to investigate the middle school and college under the jurisdiction of the municipal education commission.The qualitative data were analyzed by Chi-square test or Fisher's exact probability method for statistical tests.Results The organization and leadership of TB prevention and control work was well in all the 152 colleges and universities surveyed,but there were some deficiencies in the aspect of health care institutions and personnel equipped:93% (142/152) of the colleges and universities are equipped with a health care institutions (including the school clinic,health room,hospital,etc.) and health care workers,the ratio of the number of students and health care personnel equipped was 1 430,which was higher than the relevant state standard.The degree of full-time health care employee was mainly junior college degree (50.36%,207/411),and the proportion of medical background and qualifications of medical practitioners were 79.32% and 57.66% respectively,it remains to be improved.Although the routine TB prevention and control measures of those schools were carried out well,there were still nearly 20% of colleges and universities could not carry out student absenteeism due to illness tracking;More than 10% of them could not ensure the implement the entrance examination and nearly 30% could not ensure the implement the physical examination of the medical students.There was statistical difference in the proportion of physical examination (new students,students) between those schools (P<0.05).More than 10% of schools could not carry out close contacts screening timely after discovering a TB cases.There was no statistical difference(P>0.05).The proportion of implement medical leave/reentry management due to tuberculosis was 77.97% and have statistical significance(P<0.05),of which the proportion was obviously low in high school (55.56%).Conclusion The overall condition of TB prevention and control work in middle school and college is sound,but we still should continue to strengthen health care personnel team construction and the implement of the prevention and control measures,particularly in the aspect of physical examination,close contacts screening and leave/reentry management due to tuberculosis,to ensure the school tuberculosis epidemic smoothly.